A petition has been filed to stop any further introductions of gray wolves into Colorado.
It calls for a delay until the state finds ways to address conflicts between the already-released wolves and livestock producers.
Twenty-six organizations signed the petition calling for a clear definition of chronic depredation as well as mandated lethal take requirements.
The state is set to release more wolves this winter, a year after the first release.
The initial costs were estimated at $800,000 annually, but to date the program has cost $5 million.